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ASSAY OFAMLODIPINE AND CELECOXIB IN COMBINATION IN THE PRESENCE OF THEIR DEGRADANTS BY STABILITY INDICATING HPLC METHOD
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| Author:
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RAMA K, RAJA S
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| Abstract:
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New combination, amlodipine and celecoxib, was approved for treating patients with osteoarthritis pain and increased
blood pressure. This investigation was performed to develop and validate a stability indicting HPLC method for
simultaneous assay of amlodipine and celecoxib. Chromatographic conditions for separating and analyzing amlodipine
and celecoxib in the presence of their forced degradants was achieved by using a Kromasil C18 analytical column
(250 mm × 4.6 mm, 5 µm) and mixture of 0.1M K2HPO4:methanol in the proportion of 60:40 (v/v) with pH 6.0 as
mobile phase with a flow rate of 1 ml/min. the detection was done using photodiode array detector at 242 nm.
Regression analysis confirmed a fine correlation for amlodipine and celecoxib in the range of 5-15 µg/ml and 100-300
µg/ml, respectively. The parameters like, system suitability, selectivity, sensitivity, precision, accuracy and robustness
were validated. The values of validation parameters were satisfactory. Stability studies were carried out by exposing
amlodipine and celecoxib standard solution to different ICH stress conditions. The results showed that no
interference of degradants with the developed method.
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| Keyword:
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amlodipine, celecoxib, osteoarthritis, hypertension, HPLC analysis
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2019.11.02.057
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| Download:
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