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In-Vitro Antioxidant, Antibacterial And Anti-Inflammatory Characterization Of Indian Honey
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| Author:
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SADAF FATIMA, TOOBA NAZ SHAMSI, ROMANA PARVEEN, RIZWANA REHSAWLA, SUMBUL AFREEN, MUDSSER AZAM, TASNEEM FATMA, QAZI MOHD. RIZWANUL HAQUE, SADAF FATIMA
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| Abstract:
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Honey has been used since ages in medical and domestic need. It is said to possesswide range of medicinal properties including antimicrobial potential with lesser safety concerns. In this communication, we have evaluated antibacterial, anti-inflammatory and antioxidant potential of aqueous extract of Indian Honey. The extract was evaluated against eight bacterial pathogens including Klebsiella pneumonia (ATCC 700603), Staphylococcus aureus (MTCC 902), Bacillus subtilis (MTCC 736), Pseudomonas aeruginosa (MTCC 2453), Aeromonas species (A10 MDR), Klebsiella oxytoca (A13 MDR) and two strains of Escherichia coli (ATCC 25922 and MTCC 443). The antioxidant activity was evaluated using FRAP and DPPH assay. Also, anti-inflammatory property was analyzed by proteinase inhibitory and albumin denaturation assay. Results showed that honey completely inhibited K. oxytoca (100%) but could not inhibit B. subtilis. Honey showed DPPH radical scavenging activity in a concentration–dependent manner with maximum scavenging of 61% at 500 µl extract. The FRAP assay demonstrated highest value of 0.157 mM at 200µl of aqueous extract. The extract also showed anti-inflammatory activity by inhibiting the heat induced albumin denaturation with IC50 values 540µg/ml whereas standard drug (Aspirin) showed inhibition of 71% at 100µg/ml. Also, proteinase inhibitory assay showed minimum inhibition of 7.3% at 100µg/ml and maximum inhibition of 64% at 1000µg/ml as compared to standard which showed the maximum inhibition of 87% at 1000µg/ml. The present results reinforce the benefits of honey in the treatment of inflammatory and free radical mediated diseases.
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Antioxidant, Anti-inflammatory, Antimicrobial, Honey
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