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High Prevalence of Quinolone Drugs Resistance Genes among Clinical isolates of Shigella spp. In Al- Diwanyiah City, Iraq
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| Author:
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ABEER HAMOODI JABBAR, IBTISAM H. AL-AZAWI
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| Abstract:
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Quinolones considered one of utmost common prescribe classes for antibacterial at the world and are hired to treats a several for bacterial infection in humans. in order to wide employ (and over use ) for those antibiotics , the numbers for quinolones-resistants bacterial strains have increasing . As in case with another antibacterials agentes, the rising in quinolones resistances menace the clinical interest for that significant drugs classes. The present study included collection of stool samples from a period November 2018 until May 2019 of diarrheal patients who exhibit symptoms which includes Fever, abdominal pain , mucoid diarrhea , bloody diarrhea, of all ages, and the results of culture and biochemical tests showed 20 Shigella sp . In the present study isolates were tested to 12 antibiotics The majority of the antibiotics used in the study was resistant , also the molecular methods (Polymerase Chain Reaction ) was used in the study . Shigella isolates were investigated genotypically for harboring QR genes including . The current study recorded the highest prevalence of qnrS (95%) ),followed by qnrB(60%) , while qnrA was (55%) , then qepA in (35%) and qnrC (30%). the Phylogenetic tree analysis for nucleic acid DNA in this study was working by using the DNA Sequencing to qnrS gen because the high prevalence among isolates for this gen.
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| Keyword:
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Shigellaspp, Antimicrobialdrugs, quinolones, DNAsequencer.
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| EOI:
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-
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| DOI:
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https://doi.org/10.31838/ijpr/2020.SP1.087
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| Download:
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