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The degree of pancreatic edema in ischemic damage during acute pancreatitis in the conditions of pharmacological correction with infliximab
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| Author:
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TATIANA I. FIRSOVA, SERGEY A. ALEKHIN, ELENA N. BEZHINA, GENNADY F. LUKYANCHIKOV, KONSTANTIN M. REZNIKOV, ALEXANDER A. STEPCHENKO, GALINA A. LAZAREVA
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| Abstract:
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Introduction: Reducing tissue oxygenation is the starting point for many acute and chronic pathologies, also taking part in the deterioration of others. Ischemic damage also plays an important role in the development of pancreatic pathology, which is worsened by the development of microcirculation disorders. One of the most important reasons for the development of primary pancreatic damage and a significant increase in secondary damage is the development of interstitial edema. The development of new methods of pathogenetically based pharmacological correction of pathological processes in acute necrotic pancreatitis, based on the use of monoclonal antibodies, will significantly improve the results of treatment.
Researchtasks: the aim of our research was to study the TNFa monoclonal antibodies action on the development and value of pancreatic tissue edema in acute pancreatitis.
Material and Methods: The experiment was performed on 70 female white Wistar rats of the same age weighing 200-250g. All studies were performed in compliance with the rules of humane treatment of animals. For the study, we took rats without external signs of the disease, which passed the quarantine regime and were kept in standard conditions. All animals were divided into 5 groups. The effectiveness of infliximab in the dose range from 10 mkg/kg to 120 mkg/kg on changes in Wet/Dryratio in severe biliary necrotizing pancreatitis was studied.
Results: The use of infliximab at a dose of 10 mkg / kg 1 hour before the simulation allows to reduce the degree of edema by 1.13 ; 1.07 and 1.1 times at the corresponding study periods. The greatest protective effect of monoclonal antibodies to the tumor necrosis factor Infliximab against pancreatic edema in acute ductal necrotizing pancreatitis is expressed in a dose of 120 mkg/kg. At the time of 1 hour after the simulation, pancreatic edema was expressed 1.53 times less than in the control group, and at the time of 3 hours and 6 hours, 1.73 and 1.85 times. When compared with a dosage of 60 mkg/kg, the decrease in edema was expressed to a lesser extent when compared with the control group in 1.44; 1.62; 1.77 times at the corresponding terms.
Conclusion: Antimediative therapy including the use of infliximab is an effective method of correction for severe necrotic pancreatitis, which allows in the studied dose range to significantly reduce the degree of pancreatic edema and prevent the development of secondary necrotic process.
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| Keyword:
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acute necrotic pancreatitis, pancreatic edema, Wet/Dry ratio, infliximab.
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2020.SP1.162
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