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Benazepril and Moexipril Inhibit the Esterase Activity of Carbonic Anhydrase I and II at Micromolar Concentration, an in Vitro Investigation
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| Author:
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MOHAMMED S. M.
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| Abstract:
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Effects of Benazepril and Moexipril, which belong to angiotensin-converting enzyme inhibitor drugs on esterase activity of human Carbonic Anhydrase-I and -II, have investigated in vitro. These enzymes have purified from human erythrocytes by using the Sepharose-4B-L-tyrosine-sulfanilamide affinity gel chromatography. These enzymes have purified with specific activity 1085.1 EU/mg and 6015.38 EU/mg for human CA-I and –II respectively. The half Inhibition Concentration (IC50) values of drugs have calculated from graphs of the %100 Activity of enzymes versus [drugs]. These two drugs have shown inhibition effects on the esterase activity of human CA-I and -II at micromolar concentration. Where the IC50 values for Benazepril were 238×10-3 µM and 281×10-3µM on CA-I and –II respectively, While the IC50 values for Moexipril were 203×10-3 µM and 125×10-3 µM on human CA-I and –II respectively.
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| Keyword:
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ACE, CA-I, CA-II, Esterase activity, IC50.
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| EOI:
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-
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| DOI:
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https://doi.org/10.31838/ijpr/2020.SP1.234
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| Download:
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