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The Effect of Crab Extract on the proliferation Activity of Colon Cancer Cell Line HCT-116
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| Author:
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ALI RASIM KADHUM, ENTISAR JAWAD AL-MUKHTAR, RANA A. GHALEB, HAMID NAJI OBIED
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| Abstract:
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Background: Colon cancer is the fifth most common type of malignant growth in the world. Although treatment of cancer have good clinical outcome, chemotherapy used in treatment of cancer is still required more development. In traditional medicine, crab (Brachyura) is recommended for treatment of cancer.
Objective: Investigate the in-vitro cytotoxic effect of crab chloroform extract on colon HCT-116 cell line and normal cell line (MDCK).
Material and Methods: Crab chloroform extract was prepared in many concentrations (31.25, 62.5, 125, 250, 500, 1000 µg/mL) and assessed on HCT and MDCK cell lines over two period of incubation (24 and 48 hours) by MTT assay. Data were analyzed using a one-sample test by SPSS (version 25) one way Anova computer program and P<0.05 and P<0.001 were considered significant and highly significant respectively.
Result: In this study, it is found that HCT cells viability was significantly affected in time and dose dependent manner, thus 1000, 500, 250, 125 and 62.5 µg/mL doses showed a high significant (P<0.001) decrease in cell viability in comparison to the control group after 24 hours of incubation and 1000, 500, 250, 125, 62.5 and 31.25 doses showed a highly significant (P<0.001) decrease in cell viability after 48 hours of incubation in comparison to the control group. Occasionally normal MDCK cells viability was not significantly (P>0.05) affected by crab extract of both incubation periods (24 and 48 hours) except in high dose 1000 µg/mL after 48 hours was significant (P<0.05).
Conclusion: The decrease in cell viability of colon cancer cells and unaffected normal cells reveal that the crab extract has a selective anti-proliferative effect.
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| Keyword:
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HCT, MDCK, SPSS
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2020.SP1.268
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| Download:
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Request For Article
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