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INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

A Step Towards Excellence
Published by : Advanced Scientific Research
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0975-2366
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IJPR 9[3] July - September 2017 Special Issue

July - September 9[3] 2017

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Transmission Electron Microscopic Analysis of Zinc Oxide Nanoparticles Prepared Using Herbal Formulation

Author: VAISHNAVI DEVI. B, RAJESHKUMAR. S, DR.LAKSHMI THANGAVELU
Abstract: Background Zinc oxide nanoparticles are widely used in medical and pharmaceutical industries. They are known for their role in molecular imaging, drug delivery, diagnosis and treatment of various diseases. Zinc oxide nanoparticles synthesised using plant extract will be eco-friendly, simple and reliable methods offering therapeutic options which are safe, free of side effects and effective for a wide range of diseases. In our study we have chosen Andrographis Paniculata and Phyllanthus niruri which are widely used in Ayurveda for various systemic problems. Transmission electron microscopes can be used for understanding the characteristics of the synthesised nanoparticles. Aim The aim of the present study is to perform transmission electron microscopy analysis of zinc oxide nanoparticles prepared using herbal formulation Materials and Method Collection of Andrographis paniculata and Phyllanthus niruri plant and preparing its extract followed by the synthesis of zinc oxide nanoparticles using the prepared herbal formulation. The stable zinc oxide nanoparticles were characterised by UV- Vis spectroscopy from 300nm to 700nm. The nanoparticle solution was centrifuged and the final putrefied nanoparticle pellet is collected and stored in an airtight eppendorf tube. The nanoparticles are analysed for its morphological character using TEM. Results The zinc oxide nanoparticles prepared using the herbal formulation were characterised by UV- Vis spectroscopy, which showed shift peak at the range 350nm to 550nm. The synthesised nanoparticle had spherical shaped grain like morphology and the particle sizes distributed are in the average range of 10 - 60nm. Conclusion This simple and eco friendly biological approach for the formation of ZnO nanoparticles has a promising application in medical sciences.
Keyword: Transmission electron microscopy, Zinc oxide, Nanoparticles, Herbal formulation, Synthesis
DOI: https://doi.org/10.31838/ijpr/2020.SP1.444
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