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Evaluation of Cytotoxic Activity of Calotropis Gigantea Mouthwash-An In Vitro Study
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| Author:
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SARAVANAN , JAIGANESH RAMAMURTHY
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| Abstract:
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Calotropis gigantea plant is used in ayurvedic treatments in India in which the roots are used for asthma and barks has been used for spleen and liver disease. The plant is effective in treatment of skin, digestive, respiratory, circulatory and neurological disorders. The leaves have been used for inflammatory conditions in joints. Calotropis gigantea flowers can be prepared as extract is used as mouthwash. For that the prepared extract has to be evaluated for the cytotoxic activity. Nowadays more plant extract mouthwashes have been developed because each plant has an activity against the bacteria present in the oral cavity and it also helps to boost the immune responses against the bacteria. In India plant extract has been used in various treatments against the diseases. Ayurveda which has been practiced in India over 400Bc and this plant extract research has been done in the field of medicine in which it relieves form disease with less side effects. Calotropis gigantea which has been a native plant of India in which Calotropis gigantea flowers have been used in this study to check the mortality rate of nauplii and thereby analysing its cytotoxic nature rate of the prepared plant extract mouthwash.
Materials and methods: Calotropis gigantea leaves have been extracted from and leaves are dried for 10 days then the extracted leaves are used for mouthwash preparation. The aqueous extract of prepared leaves were used for mouthwash preparation. Mouth wash preparation was done by the aqueous extract of Calotropis gigantea .Cytotoxic assessment was done in brine shrimp. Brine shrimp were kept in fish tank with iodide free salts. Brine shrimp were added in the tank and the eggs of brine shrimp were hatched after 24 hours. Hatched Nauplii were separated and the aqueous extract of prepared vetiver mouthwash were added in different concentration of in the biologic organisms to determine the mortality rate of the prepared mouthwash in napili
Results: Cytotoxic activity test was done in aqueous extract of Calotropis gigantea to detect the mortality rate of Calotropis gigantea in which mortality rate of biologic organism were tested in napili with different concentration of 10 µl, 20 µl, 30 µl, 40 µl and 50 µl which in the mortality rate was seen in each concentration 10 µl has 70%, 20 µl has 50%,30 µl has 30%,40 µl has 20% and 50µl has 20%.
Conclusion: The above results show the cytotoxic activity of aqueous extract of Calotropis gigantea shows that the mortality rate in biologic organism. The aqueous extract of Calotropis gigantea has been studied in the field of medicine as it has high medicinal value which can be used as an alternative therapy for periodontal disease
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| Keyword:
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Calotropis gigantea, Cytotoxic activity, Nauplii, Periodontitis
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2020.SP1.430
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| Download:
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